Unraveling The Complex Relationship Between Prague, Praha

Unraveling The Complex Relationship Between

Přehled
The relationship between _Trichomonas vaginalis_, the causative agent of trichomoniasis, and lactobacilli, a group of bacteria commonly found in the vagina, is of significant interest due to its potential implications for vaginal health. It has been shown that _T. vaginalis_ is associated with a vaginal microbiota consisting of low proportions of lactobacilli and high proportions of other mostly anaerobic bacteria. _T. vaginalis_ has been found to efficiently ingest and kill lactobacilli, leading to a reduction in the lactobacilli community associated with trichomoniasis.
Our preliminary studies of proteins that are secreted by _T. vaginalis_ (secretome) upon interaction with _Lactobacillus jensenii_ pointed to several new factors with the potential to cause adverse effects on lactobacilli and to stimulate its phagocytosis.
Objective: To investigate the interactions between _T. vaginalis_ and lactobacilli _in vitro_, including the ability of _T. vaginalis_ to ingest and kill different lactobacilli species (_L. jensenii,_ _L. gasseri_, and _L. crispatus_) and investigate molecular mechanisms of trichomonas-lactobacilli interactions based on results of the preliminary study of _T. vaginalis_ secretome.
Significance: Understanding the interactions between _T. vaginalis_ and lactobacilli may provide insights into the development of targeted therapies for trichomoniasis and implications for vaginal health.
**Five relevant publications of the research group**:
Smutná T., Dohnálková A., Sutak R., Narayanasamy R.K., Tachezy J, Hrdý I. 2022. A cytosolic ferredoxin-independent hydrogenase possibly mediates hydrogen uptake in _Trichomonas vaginalis_. Current Biology, S0960-9822(21)01494-9. doi: 10.1016/j.cub.2021.10.050.
Rada P, Hrdý I, Zdrha A, Narayanasamy R.K., Smutná T., Beneš V, Tang P, and Tachezy J. 2022. Double stranded RNA viruses are released from _Trichomonas vaginalis_ inside exosomes and modulate the exosomal cargo. Frontiers in Microbiology, 13:893692. doi: 10.3389/fmicb.2022.893692.
Narayanasamy R. K., Rada P., Zdrha A., van Ranst M, Neyts J., Tachezy J. 2022. Cytidine nucleoside analog is an effective antiviral drug against Trichomonasvirus. Journal of Microbiology, Immunology and Infection, 55(2):191-198. doi: 10.1016/j.jmii.2021.08.008.
Štáfková J., Rada P., Meloni D., Žárský V., Smutná T., Zimmann N., Harant K., Pompach P., Hrdý I., Tachezy J. 2018. Dynamic secretome of _Trichomonas vaginalis_: Case study of β-amylases. Molecular and Cellular Proteomics, 2018 Feb;17(2):304-320. doi: 10.1074/mcp.RA117.000434

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